Mtt Sucks Customer Reviews and Feedback

From Everything.Sucks

The MTT assay is a colorimetric assay for assessing cell metabolic activity. NAD(P)H-dependent cellular oxidoreductase enzymes may, under defined conditions, reflect the number of viable cells present. These enzymes are capable of reducing the tetrazolium dye MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide to its insoluble formazan, which has a purple color.

An article on worldwide.promega.com discusses the limitations of the MTT assay, "The absorbance method of detection used by the MTT assay and other tetrazolium reduction assays (i.e., MTS, XTT, WST-1, WST-8) is generally less sensitive than fluorescent and luminescent methods for detecting viable cell number. This lack of sensitivity severely limits the ability to miniaturize the assay for HTS applications. Although the detection sensitivity varies widely among cell types and depends on the metabolic activity of the cell type being tested, typically tetrazolium reduction assays can detect 200–1,000 cells per well under optimal conditions. The sensitivity may be improved by optimizing the concentration of MTT and the incubation time with the cells; however, the incubation time is limited by the toxicity of the MTT reagent (10). In contrast, use of the luminescent ATP or the RealTime-Glo™ MT Cell Viability Assay to detect reducing capacity of viable cells results in detection of fewer cells per well and is more amenable to HTS applications."

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Event Manager- Front office/admin (Current Employee) says

"There are people that can complain about this company, but it all comes down to it being the right fit for people. It's a great place to work at if you like interacting with people on daily bases. The schedule can be flexible in terms of picking shifts but not flexible enough if you need a day off. Great team if you can handle office politics. Cons: Not great on benefits and compensation"

Architect (Former Employee) says

"It was a moderate atmosphere and employees were delighted to work in such work mood. MTT was located at the of the city and was convenient to most of the workers. Since it is a high-level firm, most of the projects were large-scale and demand dedication and detail researchs. Cons: Short breaks, Space"

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